Overview
Li-Brisk is a multifunctional tool for studying samples in liquid and provides an appropriate platform for integrating atomic force microscope and inverted microscope in biological research projects.
The ability of Li-Brisk to capture images in various environments along with different operation modes allows scientists to study the structure and properties of living cells and other biological samples such as DNA and RNA, proteins, viruses, bacteria, tissues, etc.
The microscope uses physical scanning for nano-imaging and sample preparation is relatively simple and does not require freezing, metal coating, vacuum, or dye injection.
Applications
- Imaging biological samples with high resolution in buffer solution.
- Investigation of the Ligand-Receptor binding.
- Investigation of intermolecular forces (force spectroscopy) in biological structures.
- Study of the unfolding of proteins.
- The possibility of performing Chemical Lithography.
- Topographical imaging down to angstrom scale resolution from live organisms.
- Nano-scale study of mechanical properties of biological Samples.
- Cutting out different sections of chromosome for genetic analysis by applying directed force.
- The Antibody-Antigen interactions studies.
Key Features
- Simultaneous use of an inverted microscope and a digital microscope during scanning.
- Easy optical adjustments by changing the laser optical path.
- Modern and easy way to tip fixation with vacuum pen.
- Accurate fixing of tip position, using Chip Alignment template.
- Optimum and easy operation, thanks to head weight reduction.
- Fast, automatic and safe approach at any tip-sample distance.
- Single LAN cable connection of the device to the computer.
- User friendly interface for the system software.
- Ability to view and save optical images in addition to nano-scanned images.
AFM and INVERTED MICROSCOPE INTEGRATION
- Biological samples are viewed from bottom neath by an inverted optical microscope. Li-Brisk model can be coupled with desired types of inverted microscopes according the the client's needs.
- Exposure from top and the possibility of adding a condenser and optical filters with different color spectrums to view all kinds of biological samples such as cells, viruses, etc.
- The possibility of viewing the inverted image as CCD output or through ocular lenses according to the user's choice.
- The possibility of being equipped with an inverted fluorescent microscope.
- Li-Brisk is equipped with objective lenses for inverted displaying samples with high resolution and magnification at scales of 4, 10. 20, and 40X.
- Easy replacement of objective lenses with user demand magnifications.
- Accurately performing focusing operation in order to obtain high-resolution images by using coarse and fine adjustments.
- Equipped with a large liquid cell (A) for imaging biological buffer matrices and other solvents.
- The minimum effect of the liquid environment on the quality and clarity of the images due to using the world's latest standards and methodologies.
- The liquid cell is made of material resistant to chemical effects, thermal changes, and environmental pH.
- Capability of simple and quick slide replacement (B) for imaging the surfaces of different samples in air.
- Newly designed scanner to reduce noise level.
- Increasing the scanning range to 50 μm and the possibility of customizing toe 100 μm range.
- The possibility to observe the opaque sample from the top during scanning using an advanced digital optical microscope.
- No need for a head while using a digital microscope for various optical analysis.
- Determination of the precise location in the scan range, accurate and fast imaging thanks to the smart design of optical parts.
Fly Kit | Pro Contact Kit | Experts Kit |
---|---|---|
Magnetic Force Microscopy (MFM) | Lateral Force Microscopy (LFM) | Chemical nano-lithography |
Electric Force Microscopy (EFM) | Force spectroscopy | Force Modulation Microscopy (FMM) |
Phase imaging | Mechanical nano-lithography | Conductive AFM (C-AFM) |
Kelvin Probe Force Microscopy (KPFM) | ||
Piezo Response Force Microscopy (PFM) |
Scanner | |
---|---|
XY scanner |
50 μm maximum XY scan range (The possibility to customize to 100 μm) 1 nm XY resolution |
Z scanner |
3 μm maximum Z movement range 0.1 nm Z resolution |
Stage | |
XY stage |
Mechanical stage 12mm travel range |
Head stage |
Mechanical XY stage: posiitoning cantilever in the center of the objective lens |
Sample mount | |
75 * 26 mm microscope slide mounting Customized 50 mm culture dish Slide and culture dish holder spring -10 V to +10 V bias voltage range to the sample |
|
Inverted Microscope | |
see table of inverted microscope items | |
AFM unit( Plug and play | |
Dimension | 580 mm * 370 mm * 600 mm |
Net weight | 20 Kg |
Head | |
High presicion adjustment micometer Optic designed for both dry and liquid environments 670 nm laser wavelength 5 mW maximum laser diode power Hige-grade quadruple photo-diode Dithering mechanism Optimized optical path design Spring lever tip holder mechanism Chip allignment mount for accurate tip mounting |
|
Head Z actuators | |
3 independent Z positioning actuator for leveling ability 15 mm travel range 40 nm movment steps Automatic engagement of the cantilever to the sample surface (Auto Fast Approach |
|
Standard modes | |
Contact Mode (Static, DC) Non-Contact Mode (Dynamic, AC) Tapping Mode (Semi-contact, Intermittent-Contact) |
|
Accessories | |
Sample mounting kit The sample substrate Various types of cantilevers Tweezers and magnet box Head-holder unit |
|
Electronics( Plug and Play control box ) | |
ADC and DAC channels |
4 channel ADC 24bit 4 channel DAC 24 bit |
Signal processing | 40 MHz frequency zynq processor |
Integrated functions | 100 MB/sec via LAN |
Software | |
Data acquisition |
Real-time 100 MB/sec Microsoft Windows compatible Integrated optical view windows for sample and cantilever vision Monitoring all system signals with a high-rated oscilloscope Auto saving captured images in the software gallery Scanning zoom-selected area on captured images Automatic fast approach of the cantilever to the sample surface (Auto Fast Approach) |
Image processing |
Independent software for image processing, data analysis, and presentation The capability of exporting different data of images Built-in with all Microsoft OS |
Dedicated all in one (AIO) computer | |
23" display monitor: 1920*1080 resolution The latest generation of processors 8 GB RAM |
|
Options | |
Top view oprical microscope |
8-megapixel resolution, color 60X to 600X optical zoom Integrated lighting Include microscope dimmer |
XY scanner |
Possibility to costumize the XY scan range to 100 μ |
Tip changing kit |
Vacuum pen |
Any requirement for specific applications or modifications can be customized.
Head | Seidentopf Trinocular Head Inclined 45°, Interpupillary Distance 48 ~ 76 mm, Light Split Switch E100:P0 /E20:P80 | |
Eyepiece | WF10/22 mm, High Eyepoint, Diopter Adjustable | |
Nosepiece | Quintuple | |
LWD Infinity Plan Objectives | LPL 4x / 0.11 | W.D. = 12.1 mm |
LPLAN 10x / 0.25 | W.D. = 8.3 mm | |
LPLAN 20x / 0.40 | W.D. = 7.2 mm | |
LPLAN 40x / 0.60 | W.D. = 3.4 mm | |
LWD Infinity Plan Fluorescent Objectives | L Plan FL 10x / 0.25 | W.D. = 10.3 mm |
L Plan FL 20x / 0.45 | W.D. = 5.8 mm | |
L Plan 40x/ 0.65 | W.D. = 5.1 mm | |
LWD Infinity Plan Phase Contrast Fluorescent Objectives Newly Update 2021 | L Plan FL PHP 20x / 0.45 | W.D. = 5.8 mm |
L Plan FL PHP 40x / 0.65 | W.D. = 5.1 mm | |
Phase Contrast | Centering Telescope 11x | |
Annular Spot |
4x 20x/ 40x 10x |
|
Working Stage | Mechanical Stage SIze 210 * 241mm, Round Slide Size Φ 110 mm | |
Condenser |
Long Working Distance, Quickly Detachable, N.A.0.3, Working Distance 72 mm (with Condenser), 195 nm (Without Condenser). |
|
Transmit Illumination |
Koehler Illumination Halogen 6V/30 W, Input voltage 100 V ~ 240V |
|
Filter | Blue, Dia. 34 mm | |
Reflect Illumination | Green, Dia.34 | |
Inverted Fluorescent | Optional |